QPNC-PAGE: The Standard Protocol
Research Paper (postgraduate) from the year 2012 in the subject Medicine - Neurology, Psychiatry, Addiction, , language: English, abstract: QPNC-PAGE (abbreviation for: quantitative preparative native continuous polyacrylamide gel electrophoresis) is a standardized variant of the electrophoresis, particularly gel electrophoresis. This analytical method of biochemistry and bioinorganic chemistry is used for the separation of charged molecules in a homogeneous electric field and allows the quantitative separation and isolation of metalloproteins from human, vegetable or animal samples with high resolution. Proteins with different molecular mass and charge are separated according to isoelelectric points and analyzed by nuclear magnetic resonance (NMR). Thus the method makes an important contribution to the structure determination of native and denatured metalloproteins and protein isomers in complex protein mixtures.
| Autor: | Kastenholz, Bernd |
|---|---|
| EAN: | 9783656292746 |
| Sprache: | Englisch Deutsch |
| Seitenzahl: | 16 |
| Produktart: | kartoniert, broschiert |
| Verlag: | GRIN Verlag |
| Untertitel: | For Acidic, Basic and Neutral Metalloproteins |
| Schlagworte: | qpnc-page Acidic Basic Neutral GelElectrophoresis homogeneouselectricfield metalloproteins isoelectricpoints complexproteinmixtures NMR protein-proteininteractions Model491PrepCell electrodereactions ExothermicReactions molecularsieving gelhomogeneity reproducibility anions cations samplematrix analytes constantspeedofelectromigration electropherogram ring-shapedproteinbands physiologicalbuffer Fractions metalproteincomplexes conformation CCS SOD-1 physiologicalconcentrations metalcofactors ICP-MS highpurity optimizedconcentration Protein-misfoldingdiseases relativebiochemicalimpact artefacts elutionprofiles timeofpolymerization newbasicprinciple standardprotocol Medicinalplants clinicalsamples |
| Größe: | 2 × 148 × 210 |
| Gewicht: | 40 g |
